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Because parameter names for any given sample must be unique, adding a second derived parameter If you wish yourĭerived parameter to be displayed on a linear scale, check the Display With Linear Scale checkbox.Įach newly-defined derived parameter is given a parameter name combining the type of function with the parameters of the function (e.g., "FL4/FL5" for ratio). You can then manually enter values for the highest and lowest number you want to display on the axis. Use the Show Range of Computed Values button to display the upper and lower limits of the parameter's values.
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Thus, you should be careful if you copy gates based on such derived parameters between samples to ensure that the gates are appropriately In some cases, this means that the same derived parameter for different samples may have different scaling. In all cases except when you add a linear ratio parameter with a defined scale, FlowJo computes the optimal scaling factors to display the most events You can have as many derived parameters as you wish (but only one time parameter can be defined). As you can see below - there is no limit to the functions you can choose from! The algebraic parameters can be created by using the pulldown Insert Parameter list (as seen above) and the Insert Function list just to its right. You can add any algebraic parameter as well as time, ratio, or log/linear conversions). When you choose to add new or edit derived parameters, you will be shown the dialog window below:įrom this window you will manage all derived parameters for a sample.